(INNC)
ALLOGENEIC STEM CELLS DERIVED FROM DONOR ADULT LIVER TISSUE. THE LIVER BIOPSY IS DIGESTED, AND THE RELEASED CELLS PLACED IN CULTURE MEDIA CONTAINING FETAL BOVINE SERUM (FBS), FIBROBLAST GROWTH FACTOR-2 (FGF- 2) AND EPIDERMAL GROWTH FACTOR (EGF). THE CELLS ARE FROZEN TO GENERATE A MASTER CELL BANK (MCB) AT AN EARLY PASSAGE, AND THEN RESUSCITATED AND CULTURE-EXPANDED. THE CELLS ARE CHARACTERIZED AS ADHERENT WITH AN ELONGATED MORPHOLOGY, THE PRESENCE (MORE OR EQUAL TO 70%) OF CELLULAR MARKERS CD29, CD73, CD90, CD105, CD44 AND ALBUMIN, AND THE ABSENCE OF CD34 (<5%), CD45 (<2%) AND CD14 (<5%). THE CELLS DIFFERENTIATE INTO FUNCTIONAL HEPATOCYTES IN A ROTARY CELL CULTURE SYSTEM IN MEDIA CONTAINING FETAL CALF SERUM (FCS), HEPATOCYTE GROWTH FACTOR (HGF), AND FIBROBLAST GROWTH FACTOR 4 (FGF4) WITH THE ABILITY TO TRANSPORT INDOCYANINE GREEN (ICG)(WHO)
BERETEMCELUM(INN)