(INNC)
ALLOGENEIC INVARIANT NATURAL KILLER T CELLS (INKT), ISOLATED FROM PERIPHERAL BLOOD LEUKAPHERESIS OF HLAA2 POSITIVE DONORS. THE INKT CELLS ARE ENRICHED USING ANTIBODY-SPECIFIC POSITIVE SELECTION, AND EXPANDED THROUGH A TWO-STEP STIMULATION PROCEDURE, INITIALLY BY CO-CULTURE WITH IRRADIATED PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) DERIVED FROM THE SAME DONOR AND ALPHA-GALACTOSYLCERAMIDE (ALPHAGALCER), AND THEN BY CO-CULTURE WITH ALPHAGALCER-PULSED IRRADIATED PBMCS, EACH IN MEDIA CONTAINING HUMAN AB SERUM AND INTERLEUKIN 2 (IL-2). THE FINAL CELL SUBSTANCE IS >99% INKTS (CD3 AND INVARIANT T CELL RECEPTOR ALPHA CHAIN POSITIVE) AND ARE IN AN ACTIVATED STATE (CD25 AND CD69 POSITIVE). THE CELLS CAN INDUCE DOSE-DEPENDENT TARGET CELL KILLING OF GLYCOLIPID-PRESENTING TUMOUR CELL LINES(WHO)
BIDOLEUCELUM(INN)