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DÉRAMIOCEL
ALLOGENEIC CARDIOSPHERE-DERIVED CELLS OBTAINED FROM CULTURED HUMAN DONOR MYOCARDIAL TISSUE. THE TISSUE IS GROWN IN CULTURE UNTIL EXPLANT DERIVED CELLS EMERGE. THESE CELLS ARE THEN FROZEN INTO A MASTER CELL BANK UNTIL SEEDED IN ULTRA-LOW ATTACHMENT FLASKS IN THE PRESENCE OF BOVINE SERUM WHERE THEY SPONTANEOUSLY FORM SPHERICAL THREE-DIMENSIONAL CELL CLUSTERS TERMED CARDIOSPHERES. THE CARDIOSPHERES ARE THEN DISRUPTED TO FORM ADHERENT SINGLE CELLS, WHICH ARE FURTHER EXPANDED ONTO FIBRONECTIN-COATED FLASKS IN THE PRESENCE OF BOVINE SERUM WITH THE CELLS UNDERGOING A RANGE OF 5-10 POPULATION DOUBLING (PDLS) TO BE CRYOPRESERVED UNTIL USE. ... PROPOSED INN: LIST 131, RECOMMENDED INN: LIST 93