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ERSÉMADROMCEL
AUTOLOGOUS MESENCHYMAL PROGENITOR CELLS PREPARED FROM SUBCUTANEOUS ADIPOSE TISSUE OF PATIENTS WITH OSTEOARTHRITIS (40-75 YEARS OLD), COLLECTED BY LIPOSUCTION. THE ADIPOSE TISSUE IS DIGESTED AND THE RELEASED CELLS PLACED IN CULTURE MEDIUM CONTAINING INSULIN-TRANSFERRIN-SELENIUM, RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH FACTOR (BFGF), AND RECOMBINANT HUMAN EPIDERMAL GROWTH FACTOR (EGF). THE CELLS ARE FROZEN, RESUSCITATED AND CULTURE EXPANDED TO AROUND PASSAGE 4-5. THE CELLS EXPRESS MESENCHYMAL PROGENITOR CELL MARKER CD73, CD90, AND CD105 (>95%) AND DO NOT EXPRESS HUMAN LEUKOCYTE ANTIGEN (HLA) DR, CD14, AND CD45 (<2%). THE CELLS ARE CAPABLE TO DIFFERENTIATE INTO CHONDROBLASTS UNDER STANDARD IN VITRO TISSUE CULTURE-DIFFERENTIATING CONDITIONS AND DEMONSTRATE THE INHIBITORY EFFECT OF MESENCHYMAL PROGENITOR CELLS ON THE PROLIFERATION OF T LYMPHOCYTES