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FAMZÉRETCEL
ALLOGENEIC HUMAN RETINAL PROGENITOR CELLS (HRPCS) DERIVED FROM FOETAL RETINA (17-20 WEEKS GESTATION). THE RETINAL TISSUE UNDERGOES DISSECTION AND DIGESTION AND THE OBTAINED CELLS ARE GROWN ON FIBRONECTIN-COATED PLATES UNDER HYPOXIC CONDITIONS IN SERUM FREE HIGH NUTRIENT MEDIA. THE FINAL CELL POPULATION IS CHARACTERIZED BY THE EXPRESSION OF RETINAL SPECIFICATION GENES (NESTIN, >95%; SOX2, >85%; KI67, >50%) AND AS WELL AS BY EVIDENCE OF DIFFERENTIATION INTO NEURONS (NEURONAL MIGRATION PROTEIN DOUBLECORTIN (DCX)) AND GLIA (GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP; <5%). THE CELLS SECRETE OSTEOPONTIN (OPN), AND THE SECRETED OPN TOGETHER WITH OTHER TROPHIC FACTORS CAN GIVE PROTECTION TO A HUMAN RETINOBLASTOMA (HRB) CELL LINE TREATED WITH A HISTONE DEACETYLASE INHIBITOR.