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ALLOGENEIC MESENCHYMAL STROMAL CELLS (MSC) DERIVED FROM SYNOVIAL DONOR TISSUE. THE PRIMARY CULTURE IS PERFORMED IN SERUM-FREE MEDIA CONTAINING LIPIDS AND CYTOKINES (E.G. EPIDERMAL GROWTH FACTOR (EGF), FIBROBLAST GROWTH FACTOR (FGF)), FOLLOWED BY EXPANSION AND HIGH DENSITY CULTURE IN MEDIA CONTAINING ADDITIONAL GROWTH FACTORS (E.G. L-ASCORBIC ACID 2-PHOSPHATE, TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA)) TO FORM EX VIVO A THREE-DIMENSIONAL TISSUE. THE FINAL CELLS EXPRESS MESENCHYMAL PROGENITOR CELL MARKERS CD13, CD44, CD73, AND CD90 (>95% EACH) AND ARE CD11B, CD34 AND CD45 NEGATIVE. THE MSCS ARE CAPABLE OF DIFFERENTIATING INTO CHONDROCYTES AND PRODUCE GLYCOSAMINOGLYCAN