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LABAFENOGEN MARSELECOBAC
LABAFENOGÉN MARSELECOBAC
LABAFÉNOGENE MARSÉLÉCOBAC
RECOMBINANT LIVE ESCHERICHIA COLI STRAIN NISSLE 1917 (ECN) BACTERIA, GENETICALLY ENGINEERED AS FOLLOWS: A) INSERTION ACROSS THE GENOME OF FOUR COPIES OF A MODIFIED PHENYLALANINE AMMONIA LYASE (MPAL) GENE FROM PHOTORHABDUS LAUMONDII SUBSP. LAUMONDII WITH MUTATIONS S92G, H133M, I167K, L432I, V470A UNDER CONTROL OF THE LACI REPRESSOR/PTAC INDUCIBLE PROMOTER. B) INSERTION OF ONE EXTRA COPY OF THE ENDOGENOUS HIGH AFFINITY PHENYLALANINE SPECIFIC PERMEASE (PHEP), UNDER CONTROL OF THE LACI REPRESSOR/PTAC INDUCIBLE PROMOTER. C) INSERTION OF THE GENE L-AMINO ACID DEAMINASE (LAAD), DERIVED FROM PROTEUS MIRABILIS, UNDER CONTROL OF THE ENDOGENOUS ARAC TRANSCRIPTIONAL REGULATOR/PBAD INDUCIBLE PROMOTER. D) DELETION OF THE DAPA GENE, WHICH RENDERS BACTERIA CELLS AUXOTROPHIC UNLESS SUPPLEMENTED WITH DIAMINOPIMELATE (DAP) EXOGENOUSLY. E) DELETION OF ITS ENDOGENOUS PROPHAGE PHI.