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RAGUNÉPROCEL
ALLOGENIC DOPAMINERGIC NEURAL PROGENITOR CELLS DERIVED FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (IPSC). THE IPSC CELL BANK WAS ESTABLISHED FROM PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) ISOLATED FROM A HEALTHY HUMAN DONOR BY EPISOMAL REPROGRAMMING WITH PLASMID VECTORS EXPRESSING OCTAMER-BINDING TRANSCRIPTION FACTOR 3/4 (OCT-3/4), SOX2, KRUEPPEL-LIKE FACTOR 4 (KLF4), L-MYC, LIN28,TUMOR SUPPRESSOR P53 (TP53), AND EPSTEIN-BARR NUCLEAR ANTIGEN 1 (EBNA1). THE CELLS WERE INITIALLY CULTIVATED WITH FEEDER-FREE STEM CELL CULTURE MEDIA WITH INTERLEUKIN-6 (IL-6), STEM CELL FACTOR, THROMBOPOIETIN, FLT-3 LIGAND, IL-3, AND GRANULOCYTE COLONYSTIMULATING FACTOR. FOLLOWING INDUCTION, THE CELLS WERE SEEDED ON PLATES COATED WITH A TRUNCATED FORM OF LAMININ IN THE SAME MEDIA. CLONES WERE CHOSEN ON THE BEST EFFICIENCY FOR SUBSEQUENT DOPAMINERGIC DIFFERENTIATION...; PROPOSED INN: LIST 126