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ALLOGENIC HUMAN ADULT NUCLEUS PULPOSUS CELLS. THE CELLS ARE DERIVED FROM THE CENTRAL REGION OF INTERVERTEBRAL DISCS AND ISOLATED FROM THE TISSUE USING MECHANICAL AND ENZYMATIC (COLLAGENASE) PROCEDURES. ISOLATED CELLS ARE EXPANDED IN CULTURE INVOLVING ADHERENT AND NON- ADHERENT CONDITIONS UNTIL CLUSTERS OF CELLS, 100- 200 MICROM IN DIAMETER, ARE GENERATED. CELLS COMPRISING THE CELL SUBSTANCE ARE DISSOCIATED OUT OF THESE CLUSTERS AND STORED FROZEN. CELLS (ON AVERAGE >95%) EXPRESS THE SURFACE MARKERS CD73, CD90, CD44 AND HLA-ABC, AND RELEASE PROTEOGLYCAN, COLLAGEN AND ANTI-INFLAMMATORY CYTOKINES. CELLS (ON AVERAGE <3%) LACK EXPRESSION OF CD24 (CLASSICALLY ASSOCIATED WITH NATIVE NUCLEUS PULPOSUS CELLS), CD34, CD45, CD271, STRO-1 OR HLA-DR/DP/DQ; REC. LIST 85