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REMEMULGÉN ARELACTIBAC
RÉMÉMULGENE ARÉLACTIBAC
NON-PATHOGENIC, FOOD-GRADE LACTIC ACID BACTERIUM LACTOCOCCUS CREMORIS (L. CREMORIS) STRAIN AUC1000, ALR DELETION DERIVATIVE OF L. CREMORIS MG1363, TRANSFORMED WITH A PLASMID ENCODING THE TRANSGENES FOR (I) HUMAN COLONY STIMULATING FACTOR 1 (CSF-1 ISOFORM 3), (II) HUMAN FIBROBLAST GROWTH FACTOR 2 (FGF-2 ISOFORM 3), AND (III) HUMAN INTERLEUKIN 4 (IL-4 ISOFORM-1) WHOSE EXPRESSION IS CONTROLLED BY A GAD OPERON CASSETTE COMPRISING THE GADR REGULATOR GENE AND A GADCB REGULATORY REGION. EACH OF THE THREE TRANSGENES IS OPTIMISED FOR EXPRESSION IN L. CREMORIS AND IS COUPLED TO THE L. CREMORIS USP45 SIGNAL SEQUENCE PRECEDED BY A RIBOSOME BINDING SITE DERIVED FROM THE GADC (CSF-1), ATPG (FGF-2) AND LACA (IL-4) GENES OF L. CREMORIS, RESPECTIVELY. THE PLASMID ALSO CONTAINS AN ALANINE RACEMASE (ALR) GENE FOR PLASMID SELECTION