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RÉPICÉRICEL
AUTOLOGOUS RED BLOOD CELLS (RBC) ISOLATED FROM WHOLE BLOOD, WITH PLATELETS REMOVED THROUGH CELL WASHING AND WHITE BLOOD CELLS (WBCS) REMOVED BY LEUKOREDUCTION FILTRATION. THE RBCS ARE RECONSTITUTED IN MEDIA CONTAINING DIMETHYLSULFOXIDE (DMSO) AND LOADED WITH ANTIGENS (SYNTHETIC LONG PEPTIDES (SLPS) CONTAINING HLA-A*02- RESTRICTED E6 AND E7 EPITOPES OF HUMAN PAPILLOMAVIRUS 16 (HPV16)) AND THE ADJUVANT POLYINOSINIC-POLYCYTIDYLIC ACID (POLY I:C), WHICH ARE DELIVERED TO THE RBCS BY FLOW THROUGH MICROFLUIDIC CHIP CONTAINING CONSTRICTIONS NARROWER THAN THE CELL THAT TEMPORARILY DISRUPTS THE CELL MEMBRANE TO ALLOW THE MATERIAL TO ENTER THE CELL. THE FINAL CELLS ARE AT LEAST 70% POSITIVE FOR GLYCOPHORIN A (CD235A) AND ANNEXIN V, AND CONTAIN POLY I:C. IN COCULTURE WITH MONOCYTE-DERIVED DENDRITIC CELLS THE RBCS INDUCE INTERFERON GAMMA (IFNGAMMA) SECRETION BY E7 ANTIGENSPECIFIC CD8+ T LYMPHOCYTES... PROPOSED INN: LIST 129, RECOMMENDED INN: LIST 91