(INNC)
ALLOGENEIC PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) ISOLATED BY APHERESIS FROM DONORS SEROPOSITIVE FOR RESPIRATORY SYNCYTIAL VIRUS (RSV), INFLUENZA A VIRUS, HUMAN METAPNEUMOVIRUS (HMPV) AND PARAINFLUENZA VIRUS (PIV). THE PBMCS ARE CO-CULTURED WITH VIRAL PEPTIDE MIXES SPANNING IMMUNOGENIC ANTIGENS FROM RSV (N, F), INFLUENZA A (NP1, MP1), PIV (M, HN, N, F), AND HMPV (F, N, M2-1, M) IN MEDIA CONTAINING HUMAN AB SERUM, INTERLEUKIN 4 (IL-4) AND INTERLEUKIN 7 (IL-7). THE FINAL CELL POPULATION CONSISTS OF MORE OR EQUAL TO 90% CD3+ T LYMPHOCYTES (MIX OF CD4+/CD8+ T LYMPHOCYTES). THE CELLS PRODUCE INTERFERON GAMMA (IFN-GAMMA) IN RESPONSE TO STIMULATION WITH RSV, INFLUENZA A, HMPV AND PIV SPECIFIC PEPTIDES. CELLS ARE STORED ACCORDING TO HUMAN LEUKOCYTE ANTIGEN (HLA) TYPE(WHO)
SPUNOLEUCELUM(INN)