(SLE)
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HUMAN DIFFERENTIATION-RESTRICTED DESCENDENTS (DRCS) OF BONE-MARROW-DERIVED ADHERENT STROMAL CELLS (MASCS) ISOLATED FROM ADULT DONOR. TO OBTAIN DRCS, MASCS WERE TRANSIENTLY TRANSFECTED WITH A DNA PLASMID ENCODING HUMAN NOTCH-1 INTRACELLULAR DOMAIN (NICD) AND EXPANDED IN GROWTH MEDIA. THE TRANSFECTION DOES NOT RESULT IN PERMANENT INCORPORATION OF THE GENE INTO THE CELLS, BUT DOES RESULT IN CHANGES IN A NUMBER OF PROTEINS AND IN THE METHYLATION PATTERN OF THE DNA (THERE IS COMPLETE LOSS OF RECOMBINANT NICD PROTEIN AND OF THE PLASMID IN THE FINAL CELL POPULATION). THE TRANSFECTION CHANGES THE NATURE OF THE CELLS SUCH THAT THEY NO LONGER READILY DIFFERENTIATE INTO BONE, CARTILAGE OR ADIPOSE CELLS, AND ALSO RESULTS IN CELLS ALTERED IN THEIR ABILITY TO SECRETE TROPHIC AND CHEMOTACTIC FACTORS, AND EXTRACELLULAR MATRIX PROTEINS TO SUPPORT DAMAGED NEURAL CELLS. RECOMMENDED INN: LIST 77(WHOM)
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